DNA—Materials & Methods
Molecular Analysis of Bacteria, Yeast and Molds
After receiving customer samples, MIDI Labs technicians extract genomic DNA from the organisms.
Amplification of the gene targets via PCR is then performed. The gene targets are 16S rRNA gene for bacteria and the D2 variable region of the Large Subunit (LSU) rRNA for fungi.
PCR products are sequenced in a double stranded manner using the latest fluorescent sequencing chemistries.
The raw sequencing data files are compiled into consensus sequences and are analyzed using Sherlock® DNA software. Identification reports based on the consensus sequence analysis are then generated.