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Molecular DNA Analysis of Bacteria Yeast and Molds

DNA—Materials & Methods

Molecular Analysis of Bacteria, Yeast and Molds

extract genomic DNA from the organisms
 

After receiving customer samples, MIDI Labs technicians extract genomic DNA from the organisms.

 

extract genomic DNA from the organisms

 

 

Amplification of the gene targets via PCR is then performed. The gene targets are 16S rRNA gene for bacteria and the D2 variable region of the Large Subunit (LSU) rRNA for fungi.

 

Fungi rRNA analysis

 

PCR products are sequenced in a double stranded manner using the latest fluorescent sequencing chemistries.

 

PCR sequencing

 

The raw sequencing data files are compiled into consensus sequences and are analyzed using Sherlock® DNA software. Identification reports based on the consensus sequence analysis are then generated.

 

DNA Analysis