DNA—Materials & Methods
Molecular Analysis of Bacteria, Yeast and Molds
After receiving customer samples, MIDI Labs technicians extract genomic DNA from the organisms.
Amplification of the gene targets via PCR is then performed. The gene targets are 16S rRNA gene for bacteria and the D2 variable region of the Large Subunit (LSU) rRNA for fungi.
PCR products are sequenced in a double stranded manner using the latest fluorescent sequencing chemistries.
The raw sequencing data files are compiled into consensus sequences and are analyzed using Sherlock® DNA software. Identification reports based on the consensus sequence analysis are then generated.
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Upcoming Events
Mandalay Bay Las Vegas, NV USA
Sponsor of Contract Laboratory Workshop on October 26th
Exhibit October 27 & October 28th, Booth #EE173
Speaker at Probiotic Workshop October 29th
Atlanta Marriott Marquis, 265 Peachtree Center Ave., Atlanta, GA USA
Exhibitor Booth # 7
Bethesda North Marriott Hotel & Conference Center, Bethesda, MD
Exhibitor Booth # 305