Fatty Acid Analysis
Bacterial identification by fatty acid analysis holds many advantages. The technology is very accurate, has a high throughput, and the cost per sample is inexpensive. Additionally, fatty acid analysis is an effective tool for strain tracking, an advantage over genetic identification systems.
MIDI Labs offers fatty acid analysis using the MIDI Sherlock® Microbial Identification System - the only automated fatty acid analysis system available. With over 750 literature references from around the world to its credit, the Sherlock® System uses gas chromatography and sophisticated pattern recognition software to identify over 1,500 species of bacteria and yeast based on the unique fatty acid methyl ester (FAME) profiles of each strain.
MIDI Labs provides strain tracking
The Dendrogram program uses cluster analysis techniques to produce unweighted pair matchings based on fatty acid compositions. The results are displayed graphically in a diagram that depicts the relatedness of pairs of entries. Multiple analyses and experience with the program have shown that species link at about 10 Euclidian Distance, subspecies at about 6 Euclidian Distance, and strain at about 4 Euclidian Distance.
Lower linkage indicates greater similarity. In the example above, none of the samples link below 3 Euclidian Distance; therefore the unknown does not belong to any of the known strains. services using the Sherlock® System's dendrogram and/or 2-D plot software, which graphically illustrate relationships between organisms.
As a sister company of MIDI, Inc., we have more experience in FAME technology and using the Sherlock® system than any other lab worldwide.
To continuously improve our services to you, the MALDI-TOF library will be updated June 2018.
This update includes:
New strains, species, and genera
Strengthened entries and increased library diversity
More than 1500 new library entries
The new library will be implemented immediately following validation and is anticipated to be available for customer samples by June 30, 2018. Please contact the Quality Assurance/Compliance Manager at QA@midilabs.com with any questions.
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