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Fatty Acid Analysis

Bacterial identification by fatty acid analysis holds many advantages. The technology is very accurate, has a high throughput, and the cost per sample is inexpensive. Additionally, fatty acid analysis is an effective tool for strain tracking, an advantage over genetic identification systems.

 

MIDI Labs offers fatty acid analysis using the MIDI Sherlock® Microbial Identification System - the only automated fatty acid analysis system available. With over 750 literature references from around the world to its credit, the Sherlock® System uses gas chromatography and sophisticated pattern recognition software to identify over 1,500 species of bacteria and yeast based on the unique fatty acid methyl ester (FAME) profiles of each strain.

 

MIDI Labs provides strain tracking

Dendrogram

The Dendrogram program uses cluster analysis techniques to produce unweighted pair matchings based on fatty acid compositions. The results are displayed graphically in a diagram that depicts the relatedness of pairs of entries. Multiple analyses and experience with the program have shown that species link at about 10 Euclidian Distance, subspecies at about 6 Euclidian Distance, and strain at about 4 Euclidian Distance. 

 

 

Fatty Acid Analysis

 

Lower linkage indicates greater similarity. In the example above, none of the samples link below 3 Euclidian Distance; therefore the unknown does not belong to any of the known strains.   services using the Sherlock® System's dendrogram and/or 2-D plot software, which graphically illustrate relationships between organisms.

 

As a sister company of MIDI, Inc., we have more experience in FAME technology and using the Sherlock® system than any other lab worldwide.