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Materials & Methods

  1. To prepare the sample for MALDI-TOF the organism is sub-cultured and incubated atoptimal growth conditions for 16-24 hours.

  2. A large, single colony or small loopful of a pure, viable culture is used for sample extraction or direct analysis.

  3. Once extracted, the sample is placed onto a target plate.

  4. Technicians overlay a matrix crystallization solution that improves spatial resolution for data analysis. 

  5. A pulsed UV laser causes the proteins to ionize toward a detector that differentiates mass based on time of flight. 

  6. The mass spectra are generated and then compared to two validated libraries.

  7. Our proprietary identification algorithm is used in generating the reports, which are then verified for quality assurance.