Materials & Methods
To prepare the sample for MALDI-TOF the organism is sub-cultured and incubated atoptimal growth conditions for 16-24 hours.
A large, single colony or small loopful of a pure, viable culture is used for sample extraction or direct analysis.
Once extracted, the sample is placed onto a target plate.
Technicians overlay a matrix crystallization solution that improves spatial resolution for data analysis.
A pulsed UV laser causes the proteins to ionize toward a detector that differentiates mass based on time of flight.
The mass spectra are generated and then compared to two validated libraries.
Our proprietary identification algorithm is used in generating the reports, which are then verified for quality assurance.
To continuously improve our services to you, the MALDI-TOF library will be updated June 2018.
This update includes:
New strains, species, and genera
Strengthened entries and increased library diversity
More than 1500 new library entries
The new library will be implemented immediately following validation and is anticipated to be available for customer samples by June 30, 2018. Please contact the Quality Assurance/Compliance Manager at QA@midilabs.com with any questions.
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